Vitroscope AS
Faktry, Leirfossvegen 5D, 7037 Trondheim, Norway. *Correspondence:
Here we describe the pre- and post-experiment cleaning protocol for vitro.alive (2D-chamber). The pre-experiment cleaning protocol is conducted right before the cell culture experiment. The post-experiment cleaning protocol is conducted right after the experiment and before storing the device in a dry place.
Preparation: Set up vitro.alive on the laboratory bench and connect it to a power supply (19 V). Insert the filling tube instead of the chamber. The device will turn on automatically and is ready to be used within 1-2 minutes. When setting up vitro.alive, keep in mind that the medium container must be within reach of the gas bottle or incubator. Also, consider whether the device needs to be within reach of an inverted microscope for live observations during the experiment.
Never push liquid through vitro.alive without the chamber or filling tube properly inserted. This will break the device.
a) Do point a) and b) in the laminar flow hood. Connect silicone tubes to the cap of the cleaning container. There should be one silicone tube for the inlet and one for the outlet.
b) Fill the container with 40 mL 75% ethanol. Close the container with the cap. Control that the inlet-tube is close to the bottom of the container.
c) Connect the cleaning container to vitro.alive by connecting the inlet-tube to “In” and the outlet-tube to “Out” on the device.
d) Connect the inlet-tube to the pump.
e) Determine the relationship between RPM and mL/min for the pump. Do this by collecting liquid for 1 minute at a chosen RPM-setting.